Aconitase
From BioComputationWiKi
Contents |
Model Authors
Fan Wu, Feng Yang, Kalyan C. Vinnakota and Daniel A. Beard
Biotechnology and Bioengineering Center and Department of Physiology
Medical College of Wisconsin
dbeard@mcw.edu
This model is described in detail in the following publication: [Read Paper Here]
and the supplemental material:
[Read Paper Here].
MathML
Reaction Definition
Aconitase is the second step in the citric acid cycle and is located between citrate synthase and isocitrate dehydrogenase. The reference chemical reaction is
The biochemical reaction, involving biochemical reactants that are sums of species, is
This model assumes a simple order uni-uni (Michaelis-Menten) reaction mechanism:
Data Used to Parameterize the Model
We have not conducted detailed studies to parameterize this model based on kinetic data. Instead, we modified published kinetic values ot account for detailed species distributions of reactants. Since the aconitase reaction is maintained near equilibrium under physiological conditions accurately simulating detailed thermodynamics is more critical than the kinetics. Data and kinetic parameters are taken from the following sources:
- Alberty, R. A. (2003) Thermodynamics of Biochemical Reactions, John Wiley & Sons, Hoboken, N.J.
- Blair, J. M. (1969) Eur J Biochem 8, 287-291 [Read Paper on PubMed]
- Kohn, M. C., Achs M. J., and Garfinkel, D. (1979) Am J Physiol 237, R159-166 [Read Paper on PubMed]
- Kohn, M. C. and Garfinkel, D. (1983) Ann Biomed Eng 11, 511-531 [Read Paper on PubMed]
- Thomson J. F., Nance S. L., Bush K. J., and Szczepanik P. A. (1966) Arch Biochem Biophys 117, 65-74 [Read Paper on PubMed]
Model Specification Sheet
Document contains model details, including data fits and parameter estimates.
All concentrations used in the flux equations presented in this sheet are free ion concentraions. To derive the free ion concentration from the total concentration, Click Here
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