NAD-Linked Isocitrate Dehydrogenase
From BioComputationWiKi
Contents |
Model Authors
Feng Qi and Xuewen Chen, Daniel A. Beard
Biotechnology and Bioengineering Center, Department of Physiology
Medical College of Wisconsin
dbeard@mcw.edu
This model is described in detail in the following publication:
Chen X. Qi F., and Beard D.A. Detailed kinetics and regulation of mammalian NAD-linked isocitrate dehydrogenase. (manuscript in review), 2008.
MathML
Reaction Definition
NAD-linked isocitrate dehydrogenase catalyzes the fourth step in the oxidation of acetyl-CoA in the citric acid cycle. The reference chemical reaction is
The biochemcial reaction, involving biochemical reactants that are sums of species, is
Data Used to Parameterize the Model
All data was taken from the following sources:
- Alberty, R.A., Thermodynamic Properties of Weak Acids Involved in Enzyme-Catalyzed Reactions. J. Phys. Chem. B, 2006. 110(10): p. 5012-5016. [PubMed]
- Portzehl, H., P.C. Caldwell, and J.C. Rueegg, The Dependence of Contraction and Relaxation of Muscle Fibres from the Crab Maia Squinado on the Internal Concentration of Free Calcium Ions. Biochim Biophys Acta, 1964. 79: p. 581-91.
- Rutter, G.A. and R.M. Denton, Regulation of NAD+-linked isocitrate dehydrogenase and 2-oxoglutarate dehydrogenase by Ca2+ ions within toluene-permeabilized rat heart mitochondria. Interactions with regulation by adenine nucleotides and NADH/NAD+ ratios. Biochem J, 1988. 252(1): p. 181-9. [Read on PubMed]
- Aogaichi, T., et al., The effects of calcium and lanthanide ions on the activity of bovine heart nicotinamide adenine dinucleotide-specific isocitrate dehydrogenase. Arch Biochem Biophys, 1980. 204(1): p. 350-6. [PubMed]
- Gabriel, J.L. and G.W. Plaut, Inhibition of bovine heart NAD-specific isocitrate dehydrogenase by reduced pyridine nucleotides: modulation of inhibition by ADP, NAD+, Ca2+, citrate, and isocitrate. Biochemistry, 1984. 23(12): p. 2773-8.
- Wu, F., et al., Computer modeling of mitochondrial tricarboxylic acid cycle, oxidative phosphorylation, metabolite transport, and electrophysiology. J Biol Chem, 2007. 282(34): p. 24525-37. [Read on PubMed]
- Tischler, M.E., P. Hecht, and J.R. Williamson, Effect of ammonia on mitochondrial and cytosolic NADH and NADPH systems in isolated rat liver cells. FEBS Lett, 1977. 76(1): p. 99-104. [PubMed]
Model Specification Sheet
Document contains model details, including data fits and parameter estimates.
All concentrations used in the flux equations presented in this sheet are free ion concentraions. To derive the free ion concentration from the total concentration, Click Here
NAD-linked Isocitrate Dehydrogenase Specification Sheet
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