Succinyl-CoA synthetase (Fan and Brian)
From BioComputationWiKi
Contents |
Model Authors
Fan Wu, Brian E. Carlson and Daniel A. Beard
Biotechnology and Bioengineering Center and Department of Physiology
Medical College of Wisconsin
dbeard@mcw.edu
This model is still under construction and the current progress is described in detail in the following file: Media:Kinetic Studies on Succinyl-CoA Synthetase.pdf.
Reaction Definition
Succinyl-CoA synthetase is a reversible "substrate-level" phosphorylation step of the TCA cycle. The enzyme is also involved heme synthesis and ketone metabolism where succinyl-CoA is required. The reference chemical reaction is
The biochemical reaction, involving biochemical reactants that are sums of species, is
There are several candidate kenetic mechanisms available (as shown in the above pdf file). In the current kinetic study is applied the order ter-ter (Michaelis-Menten) reaction mechanism (A – NTP, B – SUC, C – COAS, P – NDP, Q – SCOA, R – PI):
Data Used to Parameterize the Model
The detailed kinetic studies are still in progress. Experimental data and kinetic mechanisms are taken from the following sources:
- Alberty, R. A. (2003) Thermodynamics of Biochemical Reactions, John Wiley & Sons, Hoboken, N.J.
- Bridger, W. A. (1974) Succinyl-CoA Synthetase in The Enzymes Volume 10(edited by Boyer, P. D.), P581-606
- Cha, S., Parks, R. E. (1964) J Biol Chem, 239, P1968-1967 [Read Paper on PubMed]
- Kohn, M. C., Achs M. J., and Garfinkel, D. (1979) Am J Physiol 237, P159-166 [Read Paper on PubMed]
Model Specification Sheet
Document contains model details, including data fits and parameter estimates.
(Under Construction)
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